![]() Mechanical interpretation of force-deformation curves: available contact mechanics theorys are Hertz-Model, 'regular' Johnson-Kendal-Roberts theory, '2 point' Johnson-Kendal-Roberts theory, Derjaguin-Muller-Toporov theory, Reissner theory for thin-shell capsuls, Maugis-Dugdale theory in the approximation of Carprick and others.Determination of load and dissipated energy. Calculation of "advanced" adhesion properties based on JKR and DMT theory for different contact geometries.General processing features include: determination of baseline tilt angle and its correction to be flat "easy" calculation of the adhesion force (lowest force value while retracting) determination of the contact point: 5 different algorithm adjustable through different input values conversion from raw deflection and piezo movement to force versus deformation/separation.All systems constants like cantilever spring constant, sensitivity, probe radius etc.All curves can be processed and visualizied in graph indiviually or all at once. The procedure first indexes all force-distance curves in a specified Igor Pro Folder.Only 'name style' of the measurements must follow the principles of Asylum Research.(everything is explained in 'Naming_principles') No header-information from waves are used. Therefore, it is suitable for measurements collected at any atomic force microscope, provided the data is importable to Igor Pro. This software processes raw detector deflection signals in voltage vs raw piezo movement waves in meter.All code is commented to ease understanding the tasks and ideas behind commands used.In particular for hydrogel and polymer mechanics and adhesion, capsule mechanics and polymer brush interactions. The reliablity and variability of the code was tested and improved over a time period of ~5 years by my collegues and me in the field of soft matter research. ![]() All processes and interactions with this software are handled through a graphical user interface (GUI, see picture) to ease the handeling. I have written these IGOR Pro procedures to process, analyze and evalute collected AFM force-distance curve during my PhD thesis. The licence for the code in this repository is contained in the included license.md file. OK to reduce image resolution.An Igor Pro toolkit to process and evaluate Atomic Force Microscopy Force-Distance Measurements Note: never use software to increase pixels/µm (increase image resolution). Image visualization (MicFac does not have analysis license) High-Content Database analysis use after MetaXpress analysis Image analysis (MicFac does not have acquisition license) Scientific software, both image acquisition and image analysis Pixel-based drawing Submission of individual images for publication Vector-based drawing Image layout Submission of composite images for publication Photo software (not scientific professional/consumer grade) Some packages, such as Volocity, offer graphical programming, allowing non-programmers the ability to automate functionality. Programmable: Ability to automate functions, even add new capabilities, to package.Advanced Analysis: Various advanced functions such as tracking particle motions over time, trace neurons in 3D, select/process volumes in 3D.Basic Analysis: Calculate average brightness of whole image or of portions (rectangular/elliptical regions-of-interest, hand-traced oddly shaped regions-of-interest), scattergram plots for colocalization analysis, particle-counting (segmentation by simple thresholds).Basic Adjustments: Adjust brightness/contrast resize images crop images overlay annotations (identifying text, scale bar, etc.).This is a quick comparison of the various software choices with the following categories: We have a lot of software, both professional/consumer and scientific, that are free for users paying the instrumentation usage fee. If you're uncertain of what you're doing, don't hesitate to. Scientific image processing software is smarter and will process a whole z-stack correctly, but not necessarily associated images of control specimens. z-stack), including images of control specimens. Never use their automatic features unless you know that they do identical adjustments (quantitatively) to all images of a set (e.g. Red-eye correction of your infant nephew is fine, but red-color correction on fluorescent co-localization is not. They're great for composing images for figures, but they are extremely dangerous for novices to process scientific images. Comparison of Softwareīecause of the prevalence of point-and-shoot digital cameras, there is a lot of professional/consumer ('pro-sumer') software to adjust photographs, such as Photoshop. To take full advantage of this site, please turn it on and then refresh the page. Javascript is turned off in your web browser.
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